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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
05/10/2011 |
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Data da última atualização: |
01/02/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TISKI, I.; MARRACCINI, P.; POT, D.; VIEIRA, L. G.; PEREIRA, L. F. P. |
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Afiliação: |
CIRAD; CIRAD; CIRAD; IAPAR; LUIZ FILIPE PROTASIO PEREIRA, SAPC. |
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Título: |
Characterization and expression of two cDNA encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase isoforms in coffee (Coffea arabica L.). |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Journal of Integrative Biology/OMICS, v. 15, n. 0, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
In higher plants there are two independent pathways for isoprenoid biosynthesis, located in the cytosol (mevalonic acid or MVA pathway) or in the plastids (methylerythritol phosphate ? MEP pathway). The 3-hydroxy-3-methyglutaryl-CoA reductase (HMGR) is the first committed step in the MVA pathway. Using the information available from the Brazilian Coffee Genome Project, we found 13 ESTs that originated two isoforms, CaHMGR1 and CaHMGR2, for the enzyme HMGR of Coffea arabica. A complementary DNA encoding the isoform CaHMGR1 was cloned, and its complete nucleotide sequence determined. The full-length cDNA of CaHMGR1 was 2242 bp containing a 1812-bp ORF encoding 604 amino acids. Bioinformatic analyses revealed that the deduced CaHMGR1 had extensive homology with other plant HMGRs and contained two transmembrane domains and two putative HMGR binding sites and two NADP(H)-binding sites. Under normal growth conditions, transcripts of isoform CaHMRG1 were detected in fruit tissues (pulp, perisperm and endosperm) only at the initial stages of development, flower buds and leaves. CaHMRG2 was expressed in all tissues and during all fruit development stages examined. These results suggest a constitutive expression of isoform CaHMGR2, while the isoform CaHMGR1 shows temporal and tissue-specific transcriptional activation. |
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Palavras-Chave: |
Coffee; Expressed sequence tag (EST); Methyglutaryl-CoA reductase; Molecular characterization; Ydroxy. |
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Thesaurus Nal: |
Gene expression; Isoprenoids; Sequence analysis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/43665/1/Characterization-and-Expression-of-two.pdf
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Marc: |
LEADER 02160naa a2200265 a 4500
001 1902481
005 2013-02-01
008 2011 bl uuuu u00u1 u #d
100 1 $aTISKI, I.
245 $aCharacterization and expression of two cDNA encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase isoforms in coffee (Coffea arabica L.).$h[electronic resource]
260 $c2011
520 $aIn higher plants there are two independent pathways for isoprenoid biosynthesis, located in the cytosol (mevalonic acid or MVA pathway) or in the plastids (methylerythritol phosphate ? MEP pathway). The 3-hydroxy-3-methyglutaryl-CoA reductase (HMGR) is the first committed step in the MVA pathway. Using the information available from the Brazilian Coffee Genome Project, we found 13 ESTs that originated two isoforms, CaHMGR1 and CaHMGR2, for the enzyme HMGR of Coffea arabica. A complementary DNA encoding the isoform CaHMGR1 was cloned, and its complete nucleotide sequence determined. The full-length cDNA of CaHMGR1 was 2242 bp containing a 1812-bp ORF encoding 604 amino acids. Bioinformatic analyses revealed that the deduced CaHMGR1 had extensive homology with other plant HMGRs and contained two transmembrane domains and two putative HMGR binding sites and two NADP(H)-binding sites. Under normal growth conditions, transcripts of isoform CaHMRG1 were detected in fruit tissues (pulp, perisperm and endosperm) only at the initial stages of development, flower buds and leaves. CaHMRG2 was expressed in all tissues and during all fruit development stages examined. These results suggest a constitutive expression of isoform CaHMGR2, while the isoform CaHMGR1 shows temporal and tissue-specific transcriptional activation.
650 $aGene expression
650 $aIsoprenoids
650 $aSequence analysis
653 $aCoffee
653 $aExpressed sequence tag (EST)
653 $aMethyglutaryl-CoA reductase
653 $aMolecular characterization
653 $aYdroxy
700 1 $aMARRACCINI, P.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G.
700 1 $aPEREIRA, L. F. P.
773 $tJournal of Integrative Biology/OMICS$gv. 15, n. 0, 2011.
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Registro original: |
Embrapa Café (CNPCa) |
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| 1. |  | SANQUETTA, C. R.; BEHLING, A.; CORTE, A. P. D.; SIMON, A.; PSCHEIDT, H.; RUZA, M. S.; MOCHIUTTI, S. Estoques de biomassa e carbono em povoamentos de acácia negra em diferentes idades no Rio Grande do Sul. Scientia Forestalis, Piracicaba, v. 42, n. 103, p. 361-370, set. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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